JCDR - Brucella glycerol broth, Cryobead, Cryopreservation technique, Skim milk (2023)

JCDR - Brucella glycerol broth, Cryobead, Cryopreservation technique, Skim milk (1) Advertisers Access Statistics Resources

JCDR - Brucella glycerol broth, Cryobead, Cryopreservation technique, Skim milk (2)

Prof. Somashekhar Nimbalkar "Over the last few years, we have published our research regularly in Journal of Clinical and Diagnostic Research. Having published in more than 20 high impact journals over the last five years including several high impact ones and reviewing articles for even more journals across my fields of interest, we value our published work in JCDR for their high standards in publishing scientific articles. The ease of submission, the rapid reviews in under a month, the high quality of their reviewers and keen attention to the final process of proofs and publication, ensure that there are no mistakes in the final article. We have been asked clarifications on several occasions and have been happy to provide them and it exemplifies the commitment to quality of the team at JCDR."Prof. Somashekhar NimbalkarHead, Department of Pediatrics, Pramukhswami Medical College, Karamsad Chairman, Research Group, Charutar Arogya Mandal, Karamsad National Joint Coordinator - Advanced IAP NNF NRP Program Ex-Member, Governing Body, National Neonatology Forum, New DelhiEx-President - National Neonatology Forum Gujarat State ChapterDepartment of Pediatrics, Pramukhswami Medical College, Karamsad, Anand, Gujarat. On Sep 2018

Dr. Kalyani R "Journal of Clinical and Diagnostic Research is at present a well-known Indian originated scientific journal which started with a humble beginning. I have been associated with this journal since many years. I appreciate the Editor, Dr. Hemant Jain, for his constant effort in bringing up this journal to the present status right from the scratch. The journal is multidisciplinary. It encourages in publishing the scientific articles from postgraduates and also the beginners who start their career. At the same time the journal also caters for the high quality articles from specialty and super-specialty researchers. Hence it provides a platform for the scientist and researchers to publish. The other aspect of it is, the readers get the information regarding the most recent developments in science which can be used for teaching, research, treating patients and to some extent take preventive measures against certain diseases. The journal is contributing immensely to the society at national and international level."Dr Kalyani R Professor and Head Department of Pathology Sri Devaraj Urs Medical College Sri Devaraj Urs Academy of Higher Education and Research , Kolar, KarnatakaOn Sep 2018

Dr. Saumya Navit "As a peer-reviewed journal, the Journal of Clinical and Diagnostic Research provides an opportunity to researchers, scientists and budding professionals to explore the developments in the field of medicine and dentistry and their varied specialities, thus extending our view on biological diversities of living species in relation to medicine. ‘Knowledge is treasure of a wise man.’ The free access of this journal provides an immense scope of learning for the both the old and the young in field of medicine and dentistry as well. The multidisciplinary nature of the journal makes it a better platform to absorb all that is being researched and developed. The publication process is systematic and professional. Online submission, publication and peer reviewing makes it a user-friendly journal. As an experienced dentist and an academician, I proudly recommend this journal to the dental fraternity as a good quality open access platform for rapid communication of their cutting-edge research progress and discovery. I wish JCDR a great success and I hope that journal will soar higher with the passing time."Dr Saumya NavitProfessor and Head Department of Pediatric DentistrySaraswati Dental College LucknowOn Sep 2018

Dr. C.S. Ramesh Babu" Journal of Clinical and Diagnostic Research (JCDR) is a multi-specialty medical and dental journal publishing high quality research articles in almost all branches of medicine. The quality of printing of figures and tables is excellent and comparable to any International journal. An added advantage is nominal publication charges and monthly issue of the journal and more chances of an article being accepted for publication. Moreover being a multi-specialty journal an article concerning a particular specialty has a wider reach of readers of other related specialties also. As an author and reviewer for several years I find this Journal most suitable and highly recommend this Journal." Best regards, C.S. Ramesh Babu, Associate Professor of Anatomy, Muzaffarnagar Medical College, Muzaffarnagar. On Aug 2018

Dr. Rajendra Kumar Ghritlaharey"I wish to thank Dr. Hemant Jain, Editor-in-Chief Journal of Clinical and Diagnostic Research (JCDR), for asking me to write up few words.Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.

Authors are the souls of any journal, and deserve much respect. To publish a journal manuscripts are needed from authors. Authors have a great responsibility for producing facts of their work in terms of number and results truthfully and an individual honesty is expected from authors in this regards. Both ways its true "No authors-No manuscripts-No journals" and "No journals–No manuscripts–No authors". Reviewing a manuscript is also a very responsible and important task of any peer-reviewed journal and to be taken seriously. It needs knowledge on the subject, sincerity, honesty and determination. Although the process of reviewing a manuscript is a time consuming task butit is expected to give one's best remarks within the time frame of the journal.
Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
Timely publication of journal: Publication of manuscripts and bringing out the issue in time is one of the positive aspects of JCDR and is possible with strong support team in terms of peer reviewers, proof reading, language check, computer operators, etc. This is one of the great reasons for authors to submit their work with JCDR. Another best part of JCDR is "Online first Publications" facilities available for the authors. This facility not only provides the prompt publications of the manuscripts but at the same time also early availability of the manuscripts for the readers.
Indexation and online availability: Indexation transforms the journal in some sense from its local ownership to the worldwide professional community and to the public.JCDR is indexed with Embase & EMbiology, Google Scholar, Index Copernicus, Chemical Abstracts Service, Journal seek Database, Indian Science Abstracts, to name few of them. Manuscriptspublished in JCDR are available on major search engines ie; google, yahoo, msn.
In the era of fast growing newer technologies, and in computer and internet friendly environment the manuscripts preparation, submission, review, revision, etc and all can be done and checked with a click from all corer of the world, at any time. Of course there is always a scope for improvement in every field and none is perfect. To progress, one needs to identify the areas of one's weakness and to strengthen them.
It is well said that "happy beginning is half done" and it fits perfectly with JCDR. It has grown considerably and I feel it has already grown up from its infancy to adolescence, achieving the status of standard online e-journal form Indian continent since its inception in Feb 2007. This had been made possible due to the efforts and the hard work put in it. The way the JCDR is improving with every new volume, with good quality original manuscripts, makes it a quality journal for readers. I must thank and congratulate Dr Hemant Jain, Editor-in-Chief JCDR and his team for their sincere efforts, dedication, and determination for making JCDR a fast growing journal.
Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."

Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011

Dr. Shankar P.R. "On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal. Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement. My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did! Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review. Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors. Areas for improvement: In certain cases, I felt that the peer review process of the manuscripts was not up to international standards and that it should be strengthened. Also, the number of manuscripts in an issue is high and it may be difficult for readers to go through all of them. The journal can consider tightening of the peer review process and increasing the quality standards for the acceptance of the manuscripts. I faced occasional problems with the online manuscript submission (Pre-publishing) system, which have to be addressed. Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."Dr. P. Ravi ShankarKIST Medical College, P.O. Box 14142, Kathmandu, Nepal.E-mail: ravi.dr.shankar@gmail.comOn April 2011Anuradha Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.Dr. AnuradhaE-mail: anuradha2nittur@gmail.comOn Jan 2020

Important Notice

FAQs

Why is skim milk used in lyophilization? ›

These data suggest that a 10% skim milk freezing solution will provide greater protection against loss of viability if a freezer fails and may increase long-term survival of stocks when maintained at -80°C.

How do you freeze bacteria cultures? ›

Bacteria can be frozen using a solution of 15% glycerol. The process is simple and requires screw cap microfuge tubes and sterile glycerol. The glycerol is diluted to 30% so that it is easy to pipette. Equal amounts of 30% glycerol and culture broth are mixed, dispensed into tubes and then frozen.

Which technique is used for preservation of milk? ›

Pasteurization is the process of heating the milk at a certain temperature and suddenly cooling it to kill the microbes. At 70oC the milk is heated for 15 to 30 seconds and is immediately cooled and stored in packets. Pasteurization is the technique which can be used to preserve the food for a longer time.

Why is glycerol used to freeze bacteria? ›

Glycerol is used as a cryoprotectant as it disrupts the hydrogen bonding between water molecules, hence preventing the formation of ice crystals during freezing. This protects yeast and bacterial cells from ice crystal damage, and maintains their viability for future reculture.

What temperature does glycerol freeze? ›

when completely frozen. 0 C. -18.5 80. e Taken from literature.

Why DMSO is better than glycerol? ›

Moreover, DMSO increases the chain melting temperature,25 and glycerol leaves it unaffected. These phenomena suggest that DMSO decreases interlipid repulsion (both laterally and between bilayers), and glycerol either increases the repulsion or has little effect on the lipid–water interface.

What are the 4 steps of skimming? ›

Read the first line of each paragraph. Look for important information such as names, dates and unusual words. Read headings and subheadings. Read the conclusion or summary.

What are the 5 steps for skimming? ›

Following are some tips and techniques for recognizing what is important to read in the act of skimming.
  1. Know what you want. ...
  2. Read vertically as well as horizontally. ...
  3. Think like the author. ...
  4. Preread before you start skimming. ...
  5. Try to detect the main idea in the introductory paragraphs. ...
  6. Read the first sentence in each paragraph.
2 Dec 2020

What is skim milk also called? ›

Yes, nonfat milk (also called skim milk and fat-free milk) is a good source of protein and provides the same vitamins and minerals as whole milk — with no fat. Because the fat portion of whole milk does not contain calcium, you can lose the fat without losing any calcium.

What are the 4 types of milking methods? ›

Milking is done by using machine and generally adopted for herds with large number of cows and with high yielders. The milk flow is continuous in this method.
...
Lesson 9. METHODS OF MILKING
  • Stripping method.
  • Full hand method.
  • Knuckling method.
  • Machine milking.
29 Sept 2012

Which preservative is used in case of milk sample? ›

Alternatively, several preservative chemicals have been developed to prevent milk spoilage or preserve milk constituents, among which bronopol and azidiol are commonly used for milk chemical analysis [15].

What is the main purpose of glycerol? ›

Glycerol is used as a solvent for flavors and food colors. It is also used as a humectant, plasticizer, emollient, sweetener, and filler in low-fat food products such as cookies.

How do you store bacteria in glycerol? ›

A glycerol stock of bacteria can be stored stably at -80°C for many years and -20°C for several months. Freezing is an efficient way of storing bacteria.

How do you make glycerol broth? ›

Dilute pure glycerol in distilled water to create a 50% glycerol solution. Use a sterile pipette to measure out 10 mL of both liquids and combine them in a single flask. Stir or shake the flask thoroughly until the liquids are evenly mixed.

Does glycerol freeze easily? ›

Please note that although pure glycerin freezes at 17°C, it will typically supercool at temperatures below this, and become very thick instead of freezing. If trying to achieve acceptable performance at low temperatures an ideal mixture of glycerin/water is between 60/40 and 70/30, as can be see in the graph below.

Is glycerol soluble in cold water? ›

Hence, glycerol is soluble in water.

Is glycerol stronger than water? ›

It has been found that the hydrogen bonding ability of oxygen atom in water is stronger than that of oxygen atoms in glycerol and the hydrogen bonding ability of hydrogen atoms in water is weaker than that of hydrogen atoms in glycerol.

Why glycerol is used for preservation? ›

As a dehydrating agent, glycerol has antimicrobial and antiprotease properties and maintains corneal structure, making it suitable for long-term storage of corneas for purposes not requiring viable cell layers.

Can bacteria survive in 100% DMSO? ›

Abstract. Viable bacteria were found in six bottles of dimethyl sulfoxide (DMSO) at a concentration of approximately one bacterium per 4.4 mL. The 18 bacterial isolates appeared to be tolerating the DMSO rather than metabolizing it. No fungi were detected.

Why is glycerol used for preservation? ›

The addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria can be stored stably at -80°C for many years.

What is skim milk flocculation? ›

Skimmed milk flocculation simultaneously concentrate viruses, bacteria and protozoa. • q(RT)PCR and infectivity assay were evaluated to quantify microorganisms in water. • Uncertainty have been defined for the quantification of microorganisms.

Why is milk powder used in western blot? ›

Nonfat dry milk is commonly added to blocking buffers to minimize background noise in immunodetection procedures such as western blotting, immunofluorescence, and IHC. During western blotting, milk can be used to block non-specific binding to nitrocellulose or PVDF membranes.

Which medium is used in lyophilization? ›

Lyophilization Medium - Bacteria need a lyoprotectant which helps them survive the freeze drying process. This medium can be very simple, such as 10% skim milk, or complicated such as those that use animal sera.

What is used in lyophilization? ›

Freeze drying, also known as lyophilization or cryodesiccation, is a low temperature dehydration process that involves freezing the product, lowering pressure, then removing the ice by sublimation. This is in contrast to dehydration by most conventional methods that evaporate water using heat.

What are the properties of skim milk? ›

The composition of non-fat solids of skim milk is: 52.15% lactose, 38.71% protein (31.18% casein, 7.53% whey protein), 1.08% fat, and 8.06% ash. Fresh milk from a healthy cow is practically free from bacteria, but it must be protected against contamination as soon as it leaves the udder.

What chemical is used for flocculation? ›

Aluminum sulfate or alum (Al2(SO4)3), ferric chloride (FeCl3) and ferric sulfate (Fe(SO4)3) are the most widely used flocculants [1, 70]. These flocculants have a long history of use in removing colloidal particles from water and wastewater [15, 17].

Why is flocculation used? ›

Flocculation is a process by which a chemical coagulant added to the water acts to facilitate bonding between particles, creating larger aggregates which are easier to separate. The method is widely used in water treatment plants and can also be applied to sample processing for monitoring applications.

Why is milk a blocking agent? ›

Milk contains a lot of proteins which bind to the membrane to reduce background, non specific binding. Antibodies are proteins, so they can bind to the membrane inespecifically, this is, they bind to any site in the membrane (only due to being proteins) apart from binding to their antigen.

What is the difference between blocking with BSA and skim fat milk? ›

So the purpose of blocking step is to reduce the amount of background due to non-specific bonding. Now, BSA is only made up of one protein, BSA at 60 kDa, whereas milk is made up of many proteins, all of various sizes. So you get a much better chance to reduce more of the background banding.

Why stabilizer is used in milk? ›

Thus, stabilizing ingredients prevent product waste by thwarting premature discard of product because of undesirable appearance or mouthfeel. They keep the dairy system in place, or stabilized, through the binding of water.

Which method is used for drying? ›

Water is traditionally removed through evaporation by using methods such as air drying, sun drying, smoking or wind drying, although today electric food dehydrators or freeze-drying can be used to speed the drying process and ensure more consistent results.

Is glycerol used in lyophilization? ›

In contrast, in lyophilization glycerol is less suitable as protectants, because it can lead to an insufficiently dried and sticky product at high concentrations (Abadias et al., 2001). Sugars are another classical group of protectants used in cryopreservation and lyophilization.

What is the temperature for lyophilization? ›

Lyophilization is a time-consuming and energy-demanding process that consists of three main steps: freezing, primary drying, and secondary drying. In the first step, the product is frozen at very low temperature (around −50 °C) and most of the water solvent is converted to ice.

Which principle is used in freeze-drying? ›

The fundamental principle in freeze-drying is sublimation, the shift from a solid directly into a gas. Just like evaporation, sublimation occurs when a molecule gains enough energy to break free from the molecules around it.

What are the 3 main steps involved in freeze-drying? ›

Stages of Freeze Drying

There are three stages in the lyophilization process: Pre-freezing, primary drying, and secondary drying.

What is the principle of lyophilization? ›

The basic principle of lyophilization

Lyophilization is a process whereby a product is dried by removing the water under low temperature and pressure.

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